In vivo models adapted for Nutraceutical Research
- Myotoxicity: experimental myological alterations induced by different chemical toxicants.
- Muscle hypertrophy: treadmill running, surgical ablation of a muscle synergist.
- Muscle atrophy: denervation (chronic or transient), immobilisation, tenotomy, …
- Muscle degeneration and regeneration: several experimental procedures are available: chemical (intramuscular injection of toxins or local anaesthetics) and physical (cold, contusion, strains, exercise),..
- Exercise: chronic and acute exercise; eccentric and concentric exercise,..
- Nutrition: deprivation, hypocaloric and hypoproteic diets
Muscle ageing: study of sarcopenia (gradual loss of muscle fibres and decrease in fibre size) in several muscles.
- Histological techniques (H&E, modified Gomor trichrome, Van Giesson, toluidine blue for semithin sections,…)
- Histochemical techniques (PAS, Oil Red O, NADH-tr, myofibrilar ATPase, SDH, acid phosphatase, alkaline phosphatase, phosphorylase, ..)
- Inmunohistochemical techniques (myosin isoforms, desmin, MyoD, myogenin, etc…)
- Electron microscopic analysis: ultrastructural morphology
Morphometric analysis: muscle fibre size (area and minimum diameter), form factor, fibre types frequency, capillary density, incidence of pathologically altered muscle fibres, ultrastructural morphometry,…