IMMUNOMODULATION 

At VivaCell, we offer a wide portfolio of protocols to evaluate the natural and specific immune response in isolated human and murine lymphoid cells and also in transformed cell lines. In addition, animal models mimicking human pathologies are also offered. Thus, our “in vivo”and “in vitro”models are suitable for preclinical validation of anti-inflammatory and immunomodulatory compounds.

In vitro models

· Primary human monocytes, T-/B-lymphocytes, neutrophils, natural killer cells, dendritic cells fibroblasts, keratocytes, melanocytes, endothelial cells, chondrocytes etc.

· Murine thymocytes and primed T cells from spleen and lymphatic nodes of antigen- stimulated mice.

· Cell lines (transformed T-cell lines, B-cell lines, macrophage cell lines etc).

In vivo models
- Inflammatory Bowel Disease (IBD). A model for intestinal inflammation in swiss mice comparable to Crohn’s disease and colon ulcerative diseases. The parameters to measure are morphological and histopathological.

· Septic shock. Induced by LPS in BALB/c mice and the parameters to measure are survival and measurement of IL-1, TNFa, and IL-6 in serum. Transcription factors are also measured in isolated spleen cells of treated mice.
· Collagen-induced Arthritis (CIA). Morphological and histopathological criteria and phenotypical analysis of inflammatory infiltration in affected joints. Cytokine levels in serum.
· Experimental Autoimmune Encephalitis
· Skin allograft rejection
· Delayed-type hypersensitive reaction (murine model for human allergic contact dermatitis)
· Murine model of intestinal ischiemia-reperfusion
· In vivo activation of T and B cells (BrdU staining)
· In vivo measurement of phagocyte function.

For list of parameters, please contact us.

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