^{Recent news}

"Pharmacological inhibition of Akt and downstream pathways modulates the

expression of COX-2 and mPGES-1 in activated microglia." - Published in:
J Neuroinflammation

2012 Jan 3

^{Opinions from our customers:}

Dr. G. Weiss (PASCOE pharmazeutische Praeparate GmbH):

"We cooperate very well since many years and learned to appreciate VivaCell as a reliable and competent co-operation partner in pre-clinical research. Therefore we are looking forward to continue our successful partnership."

DERMATOLOGY / COSMETICS

hautpflege

In vitro models:
- Skin firmness:
- Measurement of adhesion molecules in cell cultures (keratinocytes, fibroblasts)
- Extracellular matrix adhesion assay
- Human skin fibroblast/collagen lattice cytotoxicity test
- Healing and migration assay:
The capacity to regenerate a “wound” in a cell monolayer culture or the capacity of a cell to migrate across a porous membrane is measured.

-Human normal keratinocyte monolayer cultures.
- Human normal fibroblast monolayer cultures.
- Red Blood Cells.
- Reconstituted human epidermis (organotypic cocultures)
Cell lines: keratinocytes (HaCaT, NCTC2544, ...), fibroblasts (MRC5, 3T3, ...), Madin Darby Canine Kidney (MDCK), etc...

Parameters:
- Cytotoxicity (MTT, Neutral Red, etc.) and membrane damage (red blood cell lysis and protein denaturation)
- Trans-epithelial permeability effect: Changes in permeability is measured as the leakage of fluorescein through an epithelial barrier (cornea, skin, confluent cell monolayer)
- Phototoxicity or protective effects of compounds: Changes in cell cultures or skin after a UV or visible light exposition:
- Oxygen radical production.
- Glutathione-S-transferase activity of cells.
- The Complement Photoactivation Assay. Complement activation can take place when human plasma and the test compound are incubated in the presence of light. The measurement of that activation is taken as an indication of potential phototoxicity of the test compound (possible contact dermatitis)

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"Hair loss-in vitro models to test 5a-Reductase Inhibition" (PDF)